Tumor-associated oncogenes go on (phage) display
نویسندگان
چکیده
منابع مشابه
Tumor-associated oncogenes go on (phage) display
www.impactjournals.com/oncotarget 84 Oncotarget 2010; 1: 84-85 The immune system has a long-recognized ability to target select proteins produced by tumor cells. Such tumor-specific antigens may be uniquely present in cancer as a result of mutations or abnormal protein modifications. In other cases, the increased immunogenicity of structurally normal proteins is less obvious and may represent, ...
متن کاملPuzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملPhage Display.
In crossing a heath, suppose...I had found a watch upon the ground, and it should be inquired how the watch happened to be in that place....[T]he inference, we think, is inevitable, that the watch must have had a maker: that there must have existed...an artificer or artificers, who formed it for the purpose which we find it actually to answer; who comprehended its construction and designed its ...
متن کاملEfficient in vivo selection of a novel tumor-associated peptide from a phage display library.
We developed a screening procedure to identify ligands from a phage display random peptide library that are selective for circulating bone marrow derived cells homing to angiogenic tumors. Panning the library on blood outgrowth endothelial cell suspension in vitro followed by in vivo selection based on homing of bone marrow-bound phage to angiogenic tumors, yielded the peptide QFPPKLTNNSML. Upo...
متن کاملEliminating helper phage from phage display
Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Oncotarget
سال: 2010
ISSN: 1949-2553
DOI: 10.18632/oncotarget.112